Figure 4. The R6H-CXCL8 mutant activated CXCR1 in neutrophils.
Human neutrophils loaded with Indo-1 were treated with and without 1 µM of the CXCR2 inhibitor SB225002 and stimulated with 100 nM of CXCL8 (A and D), the CXCR2 agonist CXCL1 (B and E) and the R6H-CXCL8 mutant (C and F). The record is representative of at least five independent experiments.