Figure 6. R6H-CXCL8 and CC-CXCL-8 mutants bound to the mutant CXCR2 containing the N-terminus of CXCR1.

COS-7 cells transfected with plasmids encoding CXCR1, CXCR2 or the NR1/CR2 (chimera in which N-terminus of CXCR2 was replaced by N-terminus of CXCR1, as described [15] were incubated with ¹²⁵I-CXCL8 (1 nM) in the absence (total binding) or presence of 100 nM unlabeled CXCL8 or 1 µM R6H-CXCL8 or CC-CXCL8. Values are means of triplicate determinations, and the bars of each point represent the standard errors.