Figure 5. EMSAs showing DNA binding activity of ORF23 to the ItA sequence nt 17348–17395 (A) and the ItB sequence nt 18580-3 (B).

The amount of purified ORF23 added in lanes 1 to 5 in (A) and (B) was 0 (−), 250 ng, 500 ng, 1000 ng, and 1500 ng, respectively. Increasing amounts of unlabeled DNA fragments (0, 0.8 pmol, 8 pmol, and 80 pmol) were incubated with 500 ng purified ORF23 protein in lanes 6 to 9 in (A) and (B), respectively.