Table 2. Oligonucleotides used in this study.
| Primer | Sequence | pMF1 coordinates |
| par-up | 5′ccggatccCGCATCGGGTGAGCGTAGAG3′ | 17242-17261 |
| par-down1 | 5′ccggaattcCCCACCCCAAAACGACAAGAGG3′ | 50-29 |
| par-down2 | 5′ccggaattcTCGCCGCAGCTTCAGCCCTC3′ | 1136-1117 |
| *parCmu-up2 | 5′CGACTCGCTCATTTCCGACCC3′ | 17427-17447 |
| *parCmu-down1 | 5′GGGTCGGAAATGAGCGAGTCGATGGTCGCGCAGGCGGTGGAG3′ | 17427-17447 17610-17630 |
| *parAmu-up2 | 5′TTCGCCAGCTCCACCGCCTGC3′ | 17618-17638 |
| *parAmu-down1 | 5′GCAGGCGGTGGAGCTGGCGAAGTCCTCTCCGCCTCAACCACC3′ | 17618-17638 18146-18166 |
| *parBmu-up2 | 5′AACCTCGGCCGGCGGGGCACG3′ | 18372-18392 |
| *parBmu-down1 | 5′CGTGCCCCGCCGGCCGAGGTTCAAATTGCCGAGGAGTTGTTCGAC3′ | 18372-18392 18555-18578 |
| **parC-u: | 5′gggaattc CATATGGGCGGGCGTCACAGGG3′ | 17392-17409 |
| **parC-d: | 5′cgcggatcc TCATCGGCCCGCCTTCTTCGC3′ | 17634-17654 |
| **parB-u: | 5′gggaattc CATATGCGTGCCCCGCCGGCCGA3′ | 17370-17388 |
| **parB-d: | 5′cgcggatcc CTACTTGGGGAGATACTTGTCGA3′ | 17574-17596 |
| DupupBiotin | 5′CGCATCGGGTGAGCGTAGAG3′ | 17242-17261 |
| Dupdo | 5′CGACTCGCTCATTTCCGACCC3′ | 17428-17447 |
| ItA | 5′GGAAGTCTGGAAGTCTAGATGTCTAGATGTTTGAATGTTTGGAGTGGG3′ | 17348-17395 |
| ItB | 5′GTGATGTCTAGAGGTCTGGATGTCTAGATGTCTAGATGTCT3′ | 18596-3 |
| ItC | 5′AGACATCTGGAAGTCTGGATGTC3′ | 13514-13537 |
| ItD | 5′AGAAGTCCAGACATCTGGACATTCAGAAGTCTAGACATCTGGATGTCCAGATGGGG 3′ | 14102-14157 |
| ItE | 5′AAGTGTCTAGATGTCTAGATGTCT3′ | 14947-14970 |
| ItF | 5′AGAGGTCTAGGTGTCTGGAAGTCTGGAGGTCTGGATGTC3′ | 15863-15902 |
| ***parC-up | 5′GGAAGAGGTCAGGGCGGCAAAG3′ | 17480-17501 |
| ***parC-down | 5′GCCTTCTTCGCCAGCTCCAC3′ | 17625-17644 |
| ***parA-up | 5′ATGATTGTCGCGGTCGTGTCCC3′ | 17651-17672 |
| ***parA-down | 5′TCCGCATCCACCACCAGCACG3′ | 17743-17763 |
| ***parB-up | 5′TCCGAGCGACCCTCAACTTG3′ | 18449-18468 |
| ***parB-down | 5′CCTCGGCAATTTGGGAGTGTTC3′ | 18546-18567 |
| 16S-up | 5′CGCCGTAAACGATGAGAA3′ | |
| 16S-down | 5′TTGCGT CGAATTAAACCAC3′ |
*primers used in gene deletion.
**primers used in gene expression in E.coli.
***primers used in quantitative PCR.