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. 2011 Dec 9;6(12):e28513. doi: 10.1371/journal.pone.0028513

Figure 3. Cell viability of cardiac adherent proliferating cells is not hampered by Coxsackievirus B3-infection.

Figure 3

CAPs were plated in a 6-well for phase contrast pictures and CVB3 copy number analysis or in a 96-well for MTS assay, respectively. After 24 h, reaching 80% confluence, CAPs were infected with CVB3 at an m.o.i. of 5 or serum starved. Four, 12, 24 and 48 h after infection or serum starvation, phase contrast pictures were taken or an MTS assay was performed. CAPs were collected 4, 12, 24 and 48 h after infection for CVB3 RNA analysis. A. Phase contrast pictures of CAPs, 4 h, 12 h, 24 h and 48 h after serum starvation (upper panel) or CVB3 infection (lower panel), at 100× magnification. B. Bar graphs representing the absorbance at 490 nm from uninfected (open bar graphs) and CVB3-infected (closed bar graphs) CAPs 4 h, 12 h, 24 h and 48 h after serum starvation or CVB3-infection, respectively. n = 6/group. C. Bar graphs representing CVB3 copy number in CVB3-infected CAPs 4 h, 12 h, 24 h, and 48 h after CVB3-infection expressed as CVB3 copy number versus human L32 (n = 3/condition) and in CVB3-infected HL-1 4 h, 12 h, and 24 h after CVB3-infection (n = 4/condition) expressed as CVB3 copy number versus human L32.