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. 2011 Dec 9;6(12):e28441. doi: 10.1371/journal.pone.0028441

Figure 8. Constitutively active CREB enhances CaRE3 but not CaRE1 activity.

Figure 8

(A) Promoter IV- or CRE-luciferase reporter construct was co-transfected with TK-pRL and VP16-CREB expression plasmid into DIV 9 neurons. (B) CaRE1- or CaRE3-luciferase reporter construct was co-transfected with TK-pRL and VP16-CREB expression plasmid into DIV 9 neurons. The activity of promoter IV, CRE, CaRE1, and CaRE3 was determined by dual luciferase assay on DIV 11. After normalized to Renilla luciferase activity, CRE, or CaRE1 or CaRE3 activity was further normalized to SV40 activity in neurons transfected with SV40-luciferase, TK-pRL, and VP16-CREB. ns: not statistically significant. Data were collected from 5 independent preparations for each treatment.