Figure 2. Smyd1b_tv1^myc and Smyd1b_tv2^myc show dynamic localizations during muscle cell differentiation in zebrafish embryos.

A–D. Whole-mount immunostaining with anti-myc antibody shows the cytosolic localization of Smyd1b_tv1^myc (A, C) or Smyd1b_tv2^myc (B, D) in myoblasts of transgenic zebrafish embryos at 14 and 24 hours-post-fertilization (hpf), respectively. A and B, dorsal views. C and D, side views. E, G, I, K. Immunostaining with anti-myc antibody shows the sarcomeric localization of Smyd1b_tv1^myc in myofibers of transgenic fish embryos at 27, 30, 48, and 72 hpf, respectively. F, H, J, L. Immunostaining with anti-myc antibody shows the cytosolic (F, H, J) and sarcomeric (L) localization of Smyd1b_tv2^myc in myofibers of transgenic fish embryos at 27, 30, 48, and 72 hpf, respectively. Scale bars: 30 µm.