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. 2011 Dec 9;6(12):e28524. doi: 10.1371/journal.pone.0028524

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A and B. Immunostaining using anti-myc antibody shows the sarcomeric localization of Smyd1b_tv1^myc on longitudinal sections of skeletal muscles from adult transgenic zebrafish expressing a myc-tagged Smyd1b_tv1 (A) or non-transgenic control (B). C–E. Double immunostaining with anti-myomesin and anti-myc antibodies shows the colocalization of Smy1b_tv1^myc with myomesin on the M-lines. F–H. Double immunostaining with anti-α-actinin and anti-myc antibodies shows the non-overlapping localization of Smy1b_tv1^myc with α- actinin. Scale bars: A = 22 µm. C = 6 µm.