Figure 1. SIM2 expression in human prostatectomy, prostate normal and cancer cell lines.

Quantitative Expression of SIM2 short isoform (A) and SIM2 long isoform (B) were evaluated by branched DNA technique in 10 normal and 14 human cancer prostatectomy specimens. Data were quantified using ALSA1 and HPRT as the normalizers. (C) Quantification of SIM2 short and long isoforms' expression in human prostate normal and cancer cell lines by real time RT-PCR. Data were quantified by the ΔΔC_T method and normalized to GAPDH. Column in white represents SIM2 short isoform and column in gray represents SIM2 long isoform. (D) Western blot were performed in prostate normal and cancer cell lines for SIM2s.