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. 2011 Dec 9;6(12):e28714. doi: 10.1371/journal.pone.0028714

Figure 6. The Rad51core promoter can be used to selectively express a toxic gene in p53-defective cells.

Figure 6

P53 defective cancer cells (as indicated) were transduced with a replication-defective Ad5 vector encoding the HSV thymidine kinase (TK) gene under the transcriptional control of the Rad51core element (Ad5ΔE1-Rad51core-TK) or the constitutively active CMV promoter (Ad5ΔE1-CMV-TK). Cells were transduced with the vector at the indicated MOIs (5 or 50), in the presence or absence of varying concentrations of ganciclovir (0–100 µM). Five days later, cell viability was determined with AlamarBlue™ dye and normalized as a percentage of the mean value of untreated cells. Data are presented as mean values of independent experimental triplicates; error bars represent the standard deviation of the data.