Figure 7. Expression of a toxic gene from the Rad51core promoter causes little toxicity in primary cells.

Primary cells (as indicated) were transduced with high doses of a replication-defective Ad5 vector encoding the HSV TK gene under the transcriptional control of the Rad51core element (Ad5ΔE1-Rad51core-TK) or the constitutively active CMV promoter (Ad5ΔE1-CMV-TK). Cells were transduced with the vector at the indicated MOIs (5–500), in the presence or absence of varying concentrations of ganciclovir (0–200 µM). Five days later, cell viability was determined with AlamarBlue™ dye and normalized as a percentage of the mean value of untreated cells. Data are presented as mean values of independent experimental triplicates; error bars represent the standard deviation of the data.