Figure 1. CBCA & CBFCA.

A) CBCA includes a permeable polycarbonate membrane and a second cell impermeable cellulose membrane directly beneath to trap attracted cells. These membranes separate the upper well containing leukocytes and the lower well containing the chemotactic. Attracted leukocytes are counted in the cellulose membrane under light microscopy in different focal planes (magnification). White arrows represent the leukocytes trapped and agglutinated into the cellulose membrane, and the yellow arrow represents its pores. Light microscopy: magnification x400; Red bar 10 µm. B) CBFCA includes a single polycarbonate membrane between the wells. Attracted leukocytes in the lower well are counted by flow cytometry and their number increases lineally with the length of interval. The leukocyte phenotype can also be determined in this step.