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. 2011 Dec 9;6(12):e28771. doi: 10.1371/journal.pone.0028771

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Gomez-Lopez, et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A&B) Two sets of assays were performed by CBCA or CBFCA, set 1 includes 12 assays (gray diamonds) and set 2 includes 15 assays (black squares). Intra-assay coefficient of variation (CV) in CBCA was higher than in CBFCA in both sets (30.1 and 17.3% vs 4.7 and 4.8%). D&C) Three of these assays were counted by three different observers/operators using microscopy or flow cytometry. The inter-observer CV in CBCA was higher than in CBFCA (30.1 vs 3.6%). Data are shown as means±SD of three independent assays counted in triplicate.