Skip to main content
. 2011 Dec 9;6(12):e28771. doi: 10.1371/journal.pone.0028771

Figure 4. A) Robustness of CBFCA.

Figure 4

To investigate the robustness of CBFCA the leukocyte chemotactic activity of undiluted and diluted plasma was determined in time course experiments. CBFCA was able to determine the biological chemotactic activity of diluted and undiluted plasma at 60, 90 and 120 min. B) Robustness of the incubation time in CBFCA. CBFCAs were performed at different incubation times (60, 90 and 120 min) using total leukocytes and plasma. All the leukocyte subsets reached the top of their migration within 90 min and their proportions did not change at 120 min of incubation time. Granulocytes and B-lymphocytes completed their migration within the first 60 min. Data shown are means±SD of five independent assays in triplicate. Means with same symbol are significantly different. Significance was determined by ANOVA and Games-Howell tests (p≤†‡γ¥ 0.0001, λ 0.005).