Skip to main content
. 2011 Dec 9;6(12):e28712. doi: 10.1371/journal.pone.0028712

Figure 2. Identification of the minimal promoter region of FPR1.

Figure 2

Serial deletion fragments of the FPR1 promoter were generated by PCR and cloned upstream from the luciferase reporter gene in the pGL3 Basic vector. 10 µg of pGL3-Control vector containing the SV40 promoter was used as positive control and 30 µg of pGL3-Basic lacking a promoter was used to measure background luminescence. The amount of pGL3-Basic-FPR1 promoter plasmids in all experiments was 30 µg. U937 cells were co-electroporated with the firefly luciferase plasmids and 300 ng of pRL-TK as a transfection standard. Results show the mean ratios of firefly to Renilla luciferase 24 hours post-transfection from 6–19 separate experiments ± S.E.M. Unpaired t test demonstrated that the luciferase activity of the −72/41 construct was significantly lower than the activity of the −88/41 construct, **p-value<0.01. Abbreviation: TSS, transcriptional start site.