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. 2011 Dec 9;6(12):e28712. doi: 10.1371/journal.pone.0028712

Figure 4. Confirmation of PU.1 binding to FPR1 promoter by ChIP-qPCR.

Figure 4

A. Cross-linked chromatin from U937 cells was sonicated to obtain an average DNA length of 600–800 bp. Immunoprecipitation was carried out using irrelevant control IgG or IgG against PU.1. The bands correspond to PCR products obtained amplifying a 324 bp fragment containing the putative PU.1 site (−87 to 237). The input DNA was obtained prior to the immunoprecipitation and represents ∼4% of the chromatin used in the immunoprecipitation. B. Cross-linked immunoprecipitated chromatin from U937 cells and human neutrophils was quantified by real-time qPCR and the amount of product was determined relative to the input chromatin. Each bar represents the mean ratio from three experiments ± S.E.M.