Figure 6. PU.1 does not bind the putative binding site in the −56/−54 SNP region of the promoter.

A. Oligonucleotide dimers of gp91^phox with a known PU.1 binding site and FPR1 with the four possible −56/−54 SNP combinations were used in EMSA. B. In vitro synthesized ³⁵S-PU.1 was incubated with gp91^phox and the various FPR1 oligonucleotide dimers. Where indicated, the incubation was carried out with a negative control (in vitro transcription/translation product using vector alone) or in the absence of oligodimer.