Figure 7. IRF1 binds the putative binding site in the −56/−54 SNP region of the promoter.

A. Oligonucleotide dimers of IRF1 consensus binding sequence and FPR1 with the four possible −56/−54 SNP combinations were used in EMSA. B. In vitro synthesized ³⁵S-IRF1 was incubated with IRF1 consensus dimer and the various FPR1 oligonucleotide dimers. Where indicated, the incubation was carried out with a negative control (in vitro transcription/translation product using vector alone) or in the absence of oligodimer. C. The binding of ³⁵S-IRF1 to the various oligodimers was quantified by densitometry of the autoradiographic films. The results show the means ± S.E.M. from three experiments. One-way analysis of variance showed that the differences in luciferase activity among the FPR1 constructs were significant (P<0.0001), and unpaired t test showed a significant difference between C/G and each of the other FPR1 SNP constructs. **p-value<0.05, ***p-value<0.001.