Table 1. Primers used for PCR.
| Organism | Fig. | Direction | Name/Gene | Applied Biosystems # | Sequence |
| Human | 1D | Forward | UFD2a | GAGCATGGATATCGATGGTG | |
| 1D | Reverse | UFD2a | GAGTGGTTCTCACCAGTTCC | ||
| 1D | Forward | HPRT | GCTGACCTGCTGGATTACAT | ||
| 1D | Reverse | HPRT | CCAGTTTCACTAATGACACAA | ||
| Mouse | 5C | UFD2a exon 9 | Mm00502528_m1 | ||
| 5C | Forward | UFD2a exon7a | Custom | TCCTCCCTGCGGATCTCT | |
| 5C | Reverse | AGTTCGAGGAAGAGGAAGGAGAAA | |||
| 5C | alpha actin | Mm00808218_g1 | |||
| 5C | MAFbx/Fbxo32 | Mm00499518_m1 | |||
| 5C | HPRT | Mm00446968_m1 | |||
| 5B 3rd??? | Forward | UFD2a exon 6 | CGAAGTCTAGACTGATTTTAAGGATCTGATTGGCC | ||
| 5B 3rd | Reverse | UFD2a exon 8 | CATATCTCGAGCACTCGATGAGGTAGTTCG | ||
| 5B 1st & 2nd | Forward | UFD2a exon 6 | CTAAGTCTAGACTTCAGCACCAGCTCGTTGTCTAG | ||
| 5B 1st | Reverse | UFD2a exon 7-8 jct* | CATTACTCGAGCAGAGGCTCCCAAACTAGGAG | ||
| 5B 2nd | Reverse | UFD2a exon 7a-8 jct | CATATCTCGAGGCTCCCAAACTGGACCCAAAC | ||
| 5B 3rd | Forward | UFD2a exon 19 | AGCTGATGCTGCGCATCCTGG | ||
| 5B 3rd | Reverse | UFD2a exon 20 | GGAGATGGAACATGTCCACGG | ||
| Zebrafish | 3C-D | Forward | UBE4B F1 | GTAACCCATTCGCCAGCCTGAC | |
| 3C-D | Reverse | UBE4B R1 | ACAGCTGGTTGGCTGCACATC | ||
| 5C | Forward | UBE4B F2 | CTCATGATGTCCACCCGTTC | ||
| 5C | Reverse | UBE4B R2 | CAGTGGGAGCGAATGTTGCTC |
*jct = junction.
panels in figure 5B are 1–3 from top to bottom.