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. 2011 Dec 9;6(12):e28894. doi: 10.1371/journal.pone.0028894

Figure 1. Gating strategy for menstrual and peripheral blood cells.

Figure 1

A. PBMC and B. MBC. Initial gating is performed from the SSC-A vs. FSC-A to determine the total lymphocyte gate (small gate) and the larger gate is used to further gate for monocytes (CD14). From the lymphocytes, live cells are gated, followed by gating B cells (CD19), T cells (CD3), CD4+CD3+ and CD8+CD3+ cells. From the CD3- gate, NK cell phenotypes are selected. CD16-CD56+(immunoregulatory cells) and CD16+CD56+(Cytotoxic cells). Percentages of positive cells for a given marker are indicated above/near gate.