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. 2011 Dec 9;6(12):e28915. doi: 10.1371/journal.pone.0028915

Figure 7. Effect of Smad2 siRNA on TGF-β1 induced collagen type I and fibronectin mRNA expression.

Figure 7

Smad2 was inhibited using the Smad2 siRNA method. Cells were transfected with Smad2 siRNA for 48 h, followed by treatment with TGF-β1 for 24 h. (A) Smad2 siRNA significantly reduced collagen type I synthesis in TGF-β1-stimulated cells. (B) Smad2 siRNA significantly reduced fibronectin synthesis in TGF-β1-stimulated cells. (C) Smad2 mRNA was decreased to 37.8% using real-time RT–PCR in Smad2 siRNA-transfected ADPKD cyst-lining epithelial cells. The results were representative of three independent experiments. *P<0.05 vs. control, # P<0.05 vs. TGF-β1 alone.