Table 1.
cDNA microarray analysis of candidate BTCC markers
| Stage Ta-T1 | Stage T2-T4 | ||||
|---|---|---|---|---|---|
| Candidate | Symbol | Median T:N fold change | %T >95th percentile of normal | Median T:N fold change | %T>95th percentile of normal |
| Bladder cancer specific nuclear matrix protein 4 | BLCA-4 | 7 | 47/51 (92.2%) | 5 | 16/21 (76.2%) |
| SMAD family member 4 | SMAD4 | 6 | 29/44 (65.9%) | 11 | 11/21 (52.4%) |
| Retinoblastoma 1 | RB1 | 3 | 32/48 (66.7%) | 3 | 17/20 (85.0%) |
| Sperm-associated antigen 5 | SPAG5 | 8 | 31/44 (81.9%) | 6 | 19/21 (90.5%) |
| Transformed mouse 3T3 cell double minute 2 | Mdm2 | 19 | 36/50 (72.5%) | 21 | 9/19 (47.4%) |
| Topoisomerase (DNA) IIa 170kDa | TOP2A | 8 | 42/50 (84.0%) | 7 | 19/21 (90.5%) |
| Homeobox A13 | HOXA13 | 27 | 45/51 (88.2%) | 31 | 19/21 (90.5%) |
| Target of methylation-induced silencing | TMS1/ASC | 4 | 27/44 (61.4%) | 4 | 13/20 (65.0%) |
| Homo sapiens zinc finger protein 85 (HPF4, HTF1) mRNA | ZNF85 | 8 | 23/42 (54.8%) | 4 | 16/21 (76.2%) |
| Insulin-like growth factor 1 | IGF-1 | 10 | 32/51 (62.7%) | 34 | 19/21 (90.5%) |
| Human telomerase reverse transcriptase | hTERT | 14 | 29/51 (87.9%) | 21 | 20/21 (95.2%) |
| Phosphatase and tensin homolog deleted on chromosome 10 | PTEN | 10 | 30/35 (85.7%) | 7 | 15/18 (83.3%) |
| Epidermal growth factor-like domain, multiple 6 | EGFL6 | 4 | 26/44 (59.1%) | 4 | 11/17 (64.7%) |
| Matrix Gla protein | MGP3 | 9 | 31/47 (65.9%) | 3 | 15/19 (78.9%) |
| Semaphorin sem2 | SEM2 | 9 | 28/44 (63.6%) | 6 | 16/20 (80.0%) |
| Biquitin-conjugating enzyme E2C | UBE2C | 11 | 14/37 (37.8%) | 44 | 13/20 (65.0%) |
| Human mRNA for highly conserved cyclin family | cyclin D2 | 5 | 37/50 (74.0%) | 3 | 11/17 (64.7%) |
| Baculoviral IAP repeat-containing 5 (survivin) | BIRC5 | 9 | 19/46 (41.3%) | 28 | 3/20 (15.0%) |
| Ras association domain family 1 Agene | RASSF1A | 22 | 37/49 (75.5%) | 6 | 9/20 (45.0%) |
BTCC: bladder transitional cell carcinoma; hTERT: human telomerase reverse transcriptase; BLCA-4: bladder cancer specific nuclear matrix protein 4; HOXA13: homeobox A13.