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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Sep 6;36(2):214–222. doi: 10.1111/j.1530-0277.2011.01607.x

Fig. 3.

Fig. 3

Chronic ethanol feeding decreased GSH/GSSG ratio in high density microsomes isolated from adipose tissue, associated with an increase in CYP2E1 expression. Rats were allowed free access to an ethanol-containing diet or pair-fed a control diet for 4 weeks. Epididymal adipose tissue was homogenized, high density microsomes were isolated and lysed. (A) GSH/GSSG, (B) GSH, (C) GSSG, and (D) total glutathione were determined by a HPLC-based method as described in methods. n=6. (E) Immunoreactive CYP2E1 was measured by western blot in high density microsome lysates. n=4–5. Images are representative of 4–5 rats per treatment group. Values represent means ± SEM. Values with an asterisk are significantly different from each other, p< 0.05.