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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Sep 6;36(2):214–222. doi: 10.1111/j.1530-0277.2011.01607.x

Fig. 7.

Fig. 7

4-HNE induced ER stress in 3T3-L1 adipocytes. Mature 3T3-L1 cells were cultured in media with or without 50 or 100 μM 4-HNE at 37°C for up to 4h. (A) Immunoreactive phospho-eIF2α and total eIF2α were detected by western blot. A representative image from 3 independent experiments is shown. (B) CHOP and Grp78 mRNA level in 3T3-L1 adipocytes were measured by RT-PCR and then normalized to 18S mRNA level. n=6. Values represent means ± SEM. Values with different superscripts (A) or asterisks (B) are significantly different from each other, p< 0.05.