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. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: Oncogene. 2011 Sep 5;31(14):1804–1816. doi: 10.1038/onc.2011.361

Figure 5. c-Abl promotes invasion in a STAT3-dependent manner.

Figure 5

Invasion assays were performed on: (a) 435s/M14 cells transfected with scrambled or STAT3 siRNAs; (c) 435s/M14 cells stably expressing vector or STAT3C, transfected with c-Abl/Arg siRNAs; (d) 435s/M14 cells stably expressing GFP or GFP-MMP-1, transfected with c-Abl/Arg siRNAs; and (e,f) 435s/M14 cells, transfected with c-Abl or Arg siRNAs and incubated with recombinant MMP-1 or MMP-3 (25 ng/ml) during the invasion assays. Photographs are at 100X magnification. The total number of invaded cells on the undersurface of the membranes were counted and expressed as a percentage of scrambled. Graphs are Mean ± S EM, n=3 independent experiments, performed in duplicate. *0.01≤p<0.05; **0.001≤p<0.01; ***p<0.001. Some error bars are too small to be visualized. (b, d-top) Knockdown efficiency and MMP-1 expression in stably expressing cells were determined by semi-quantitative RT-PCR (35, 27 cycles, respectively); representative experiments are shown.