. Author manuscript; available in PMC: 2012 Oct 19.

Published in final edited form as: Oncogene. 2011 Sep 5;31(16):2028–2038. doi: 10.1038/onc.2011.385

Table 1.

Quantitative Real Time PCR for Apc expression levels in the small intestine

	Apc	Actin and GAPDH	Δ C_T	ΔΔ C_T	% Apc^+/+
	C_T	C_T	Δ C_T	ΔΔ C_T	% Apc^+/+
*Apc^+/+*	25.48	14.07	11.41	0	100
*Apc^CKO/CKO*	24.11	12.71	11.40	0.01	101
*Apc^580S/580S*	26.27	13.53	12.74	−1.33	40

C_T = cycle threshold (same threshold level of fluorescence for all samples)

ΔC_T = C_T (Apc) − C_T (actin and GAPDH)

ΔΔC_T = ΔC_T (Apc^+/+) − ΔC_T (Apc^CKO/CKO or Apc^580S/580S)

%Apc^+/+ = 2^ΔΔCt × 100