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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: BJU Int. 2011 Aug 26;109(6):949–958. doi: 10.1111/j.1464-410X.2011.10408.x

Fig. 3.

Fig. 3

Levels of pro-caspase-3, pro-caspase-8, and pro-caspase-9 levels were assessed via western blots after freezing alone (A and B) or VD3 and freeze treatment combination (C and D) in AS and AI samples for a 24-h period after freezing. The AS cells (A) exhibited greater control levels of each caspase than AI samples (B). Compared to freezing alone, levels of pro-caspase-3 and pro-caspase-8 after the combination of VD3 and freezing were found to be reduced in all cell lines evaluated, indicating increased caspase activation (C and D). AI samples exhibited decreases in pro-caspase expression (especially pro-caspase-9) which was similar to AS samples (D and A vs B) indicating that VD3 was able to induce similar apoptotic effects in response to freezing in all samples tested.