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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: BJU Int. 2011 Aug 26;109(6):949–958. doi: 10.1111/j.1464-410X.2011.10408.x

Fig. 6.

Fig. 6

Tissue-engineered matrices containing untreated or VD3-treated AS and AI HP cells were frozen using a single freezing cycle (A and B) or a double freezing cycle (C and D). Matrices were frozen for a single or double freeze followed by return to 37°C. Twenty-four hours after thawing, matrices were probed with calcein AM (green, live cells) and propidium iodide (red, dead cells). A 50× panoramic series of fluorescent micrographs was taken extending from the centre near the cryoprobe tip (left of images) to the periphery of the ice sphere (right of image).