Figure 1.

CLSM images of biofilm on soft tissue from patient 1. Overlay projection (includes all the slices in an image stack) of the biofilm at the center of the ulcer base (A) and at the edge of the ulcer base (B). Bar represents 75 µm. CLSM examination revealed the presence of bacteria ranging from single cells to large aggregates of grape-like clusters (panel A). The bacteria in these clusters were viable, as they appeared fluorescent green after LIVE/DEAD Baclight viability stain. Calcofluor white (blue) stained the EPS excreted by the bacteria (panel B). Host nuclei and fibrous material stained red with propidium iodide (panels A and B).