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Time dependent fluorescence intensities (I) of 4 (λex=360 nm, λem = 465 nm) formed by CuAAC reaction of CPL (1 mg mL−1) with azide 3 (5 mM) in the presence of (A) 312.5 μM Cu2+, 6.25 mM ascorbic acid and 625 μM ligand 5, and (B) 2 mM Cu2+, 50 mM ascorbic acid.