FIG. 4.

Cell proliferation in the perihematomal areas 10 days after ICH. Typical hemorrhagic lesion is shown in a normotensive rats (A) and a RVHT rat (B). L, left hemisphere; R, right hemisphere. Cells double-labeled with BrdU and nestin are counted in four randomly selected grids (black boxes show examples) at the edge of the hematoma (A,B). Representative microscope images show proliferating cells on the edge of the hemorrhagic lesion in a normotensive rat (C) and a RVHT rat (D). Scale bar, 100 μm (C,D). Quantitative analysis of BrdU positive cells on the edge of hematoma using an unbiased stereological manner reveals that the RVHT rats have a greater density of proliferating cells than the normotensive rats (E). *p<0.01 versus normotensive group. Double-staining with BrdU and nestin in the perihematomal areas reveals that the majority of BrdU positive cells are also nestin positive. Note double-labeling (white arrows) for BrdU and nestin in a normotensive rat (F1–F3) and a hypertensive rat (G1–G3). Scale bar, 70 μm in F1–F3 and G1–G3. Color image is available online at www.liebertonline.com/neu