Fig. 8.

a–b ¹H-NMR spectrum obtained by postacquisition coaddition of the cITP-NMR spectra of 2.5 μg of the pure heparin oligosaccharide. a. The four anomeric protons marked with asterisks indicate that the unknown oligosaccharide is a tetrasaccharide. ¹H NMR spectrum of the heparin tetrasaccharide acquired using the CapNMR probe with 30 μg of the oligosaccharide in the probe flowcell. b. Enlarged portion of the spectrum shows the characteristic d-glucuronate H-4 triplet at 3.38 ppm. Adapted from [137] with permission