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. Author manuscript; available in PMC: 2012 Dec 13.
Published in final edited form as: Biochemistry. 2011 Nov 14;50(49):10687–10697. doi: 10.1021/bi201426b

Figure 5. Immunization of 2×Tg mice with oxidized Met-rich protein reduces Msr activity and brain Aβ burden.

Figure 5

Mice were immunized for 6-months with oxidized Met-rich protein (DSZ18(ox)) or adjuvant alone. Non-Tg mice served as a negative control and unimmunized mice as a positive control. N = 5 mice per group. A) Measurement of specific Msr activity in mouse brain. ***p < 0.001. NS – non-significant. B) Westren blot analysis for detection of anti-Met(O) antibodies in sera of 2×Tg mice immunized with DZS18(ox), mice immunized with adjuvant alone, or unimmunized mice. The smear between 45–60 kDa in the 2×Tg + DZS18(ox) lane is an artifact that was not observed in other blots. C–E) Fluorescence microscopy images of hippocampal brain slices stained with Thioflavin S: C) Unimmunized mice. D) Mice immunized with DZS18(ox). E) Mice immunized with adjuvant alone. F) % Plaque burden was quantified by calculating the total ThS-stained area divided by the total hippocampal area measured and normalized to unimmunized mice using ImageJ (n = 5 mice per condition, *p < 0.05).