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. 2011 Nov 30;34(1):167–174. doi: 10.1007/s10529-011-0750-1

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© The Author(s) 2011

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Fig. 2 — Overexpression of MsHSP23 in E. coli. SDS-PAGE analysis of the recombinant protein expressed in E. coli. Lane 1 BL21 total protein, lane 2 pET28a total protein (−IPTG), lane 3 pET28a total protein (+IPTG), lane 4 MsHSP23 soluble protein Induction 1 h (+IPTG), lane 5 MsHSP23 soluble protein Induction 3 h (+IPTG), lane 6 MsHSP23 soluble protein Induction 5 h (+IPTG), lane 7 MsHSP23 soluble protein Induction 7 h (+IPTG), lane 8 MsHsp23 soluble protein Induction 21 h (+IPTG), lane 9 MsHSP23 soluble protein Induction 24 h (+IPTG)