Table 1. Primers used for PCR.
| Exon | Forward (5′-3′) | Reverse (5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| 4 |
TGCAGCTGCCCGAGGATTA |
GCACCCCGAGCCCGAAGTC |
144 |
66 |
| 5 |
TCCCTCTTCTTCCTCTTCACT |
GGGGTCCATAATTAGCATC |
301 |
58 |
| 5 a,6 |
GCTCTCTACAGTAAGTTCTC |
AGGAGAGAGCATTGGGCTTA |
457 |
59 |
| 7 |
AATCCACCCACTGTCCCG |
CCAGCCACCTTCATACCG |
542 |
60 |
| 8 |
TCAGGTAACTAACATCGCA |
GTTGACTGTACTTGGAAGAA |
719 |
53 |
| 9,10,11 |
GAGGTGGGAACCAGTTTGATG |
CAAGCCAATCTCTGTAGTGCG |
890 |
52 |
| 12 |
GCTGTGTGATGTGTTCCTCA |
AAGAGAGATCGCCTCTGTG |
245 |
58 |
| 13 | CATGTCTGTTTCTCAAAGGG | CCATAGTCACTGACTGAATTAACAC | 202 | 58 |
Summary of the primers and product length used for the amplification of PAX6 exons.