Figure 1.

Comparative immunohistochemical analysis of dystrophin expression in 17 patients with Becker muscular dystrophy with in-frame deletions. Control, Duchenne muscular dystrophy and Becker muscular dystrophy transverse muscle sections were immunolabelled for β-spectrin and with MANDYS106 (exon 43) and Dys2 (C-terminal, last 17 amino acids) antibodies against dystrophin. Expression was quantified relative to control muscle in 40 muscle fibres and normalized to β-spectrin expression. Values represent means ± SEM except for the bottom graphs where values represent the mean expression level for each group ± SD of the difference between sample means. In the bottom two graphs patients with Becker muscular dystrophy were grouped according to corresponding exon skipping models for Duchenne muscular dystrophy: exon 51 skipping (Model 51, red bars), multi-exon skipping (model MS, blue bars) and exon 53 skipping (Model 53, yellow bars).