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Effect of nitrogen source on Bacillus sp. strain CR-179 growth and protease activity. The culture density and extracellular protease activity were determined during 24 h incubation at 45°C and at initial pH 8.
| Nitrogen source | Culture density (OD 600 nm) | Maximum enzyme activity (U/mL) |
|---|---|---|
| Corn steep liquor | 1.410 | 336.233 |
| Whey protein | 1.114 | 239.870 |
| Beef extract | 1.383 | 203.246 |
| Yeast extract | 1.391 | 308.961 |
| Tryptone | 0.954 | 190 |
| Peptone | 1.030 | 119.350 |
| Urea | 0.666 | 46.883 |
