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Effect of pH on growth and protease activity by Bacillus sp. strain CR-179 cultivated in a liquid medium containing starch (1%) and corn steep liquor (0.4%) in shake flasks during 24 h at 45°C.
| Initial pH | Culture density (OD 600 nm) | Maximum enzyme activity (U/mL) |
|---|---|---|
| 6 | 0.980 | 168.18 |
| 7 | 1.335 | 260.129 |
| 8 | 1.402 | 335.454 |
| 9 | 1.012 | 253.37 |
| 10 | 0.734 | 163.506 |
