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Effect of agitation rate on growth and protease activity by Bacillus sp. strain CR-179 cultivated in a liquid medium containing starch (1%) and corn steep liquor (0.4%) in shake flasks during 24 h at 45°C.
| Agitation rate (rpm) | Culture density (OD 600 nm) | Maximum enzyme activity (U/mL) |
|---|---|---|
| 110 | 1.327 | 301.688 |
| 130 | 1.388 | 320.389 |
| 150 | 1.418 | 345.844 |
| 180 | 1.330 | 307.142 |
