Aspirin and SOX7 inhibit the growth of SW480 cells. A: Dose- and time-dependent effects of aspirin on the growth rates of SW480 human colon cancer cells. SW480 cells were treated for 24, 48 or 72 h in aspirin-containing culture medium; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to estimate the survival percentage compared with untreated cells; B: Effect of SOX7 expression on cell proliferation. MTT assay was used to estimate the proliferation at different time points after transfection of SOX7 expression vector; C: Western blotting was used to verify the expression of SOX7 after transfection of SOX7 expression vector at indicated time points. MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. ASA: Aspirin.