Figure 4.

Role of the p38MAPK pathway in aspirin-induced SOX7 expression. A: HEK 293T cells were transfected with p38α expression vector; reverse transcription-polymerase chain reaction (RT-PCR) was used to detect SOX7 mRNA level 30 h after transfection; B: SW480 cells were treated with 10 μmol/L SB203580 for 30 h and SOX7 mRNA level was measured by RT-PCR; C: SW480 cells were treated with aspirin or aspirin combined with SB203580 for 30 h and SOX7 mRNA level was measured by RT-PCR; D: Cells were treated as in Figure 4 and SOX7 protein level was detected by Western blot; E: SW480 cells were transfected with pGL3-SOX7-luc plasmid and various expression vectors as indicated for 24 h and relative luciferase activity was examined. ASA: Aspirin.