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. Author manuscript; available in PMC: 2011 Dec 12.
Published in final edited form as: Sci Transl Med. 2010 Nov 10;2(57):57ra82. doi: 10.1126/scitranslmed.3001510

Figure 4. TLR9 Expression in Rapid and Slowly Progressive IPF Lung Fibroblasts and Response to CpG.

Figure 4

A. qRT-PCR analysis of TLR9 gene expression in representative rapid UIP/IPF (n=5-8) (a) and slow IPF (n=5-8) (b) fibroblast cell lines treated for 24 h without (untreated) or with CpG-ODN (10 μg/ml) in the presence or absence of IL-4 (10 ng/ml). Fold increase is calculated within each group of disease compared with the respective untreated fibroblasts. Bioplex analyses of rapid or slow IPF fibroblast conditioned media for IFNα (c and d), PDGF (e and f), MCP-1/CCL2 (g and h), and MCP-3/CCL3 (i and j). Fibroblast cell lines were treated for 24 h without (untreated) or with CpG-ODN (10 μg/ml) in presence or absence of IL-4 (10 ng/ml). Data is representative of at least 5 slow IPF and 5 rapid IPF fibroblast cell lines. Data are mean ± SEM. ** p<0.001 and *** p < 0.0001.