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. Author manuscript; available in PMC: 2012 Dec 13.

Published in final edited form as: Biochemistry. 2011 Nov 14;50(49):10647–10657. doi: 10.1021/bi201619z

Table 2.

Stabilization of GCase with inhibitors at acidic and neutral pH.

		1		2		3		IFG
	Inhibitor (mM)	T_m(°C)	ΔT_m	T_m(°C)	ΔT_m	T_m(°C)	ΔT_m	T_m(°C)	ΔT_m
pH 5.2		55.8 ± 0.0^a
	0.5	56.4 ± 0.0	0.6	55.9 ± 0.1	0.3	56.1 ± 0.0	0.3	68.0 ± 0.2	12.2
	1	56.6 ± 0.0	0.8	56.0 ± 0.0	0.3	56.1 ± 0.1	0.2	68.9 ± 0.3	13.0
	2	57.3 ± 0.1	1.5	56.7 ± 0.1	1.0	56.7 ± 0.1	0.8	69.7 ± 0.3	13.8
	5	58.4 ± 0.1	2.7	57.4 ± 0.1	1.7	57.2 ± 0.1	1.3	67.5 ± 0.3	11.6
	10	59.0 ± 0.1	3.2	57.4 ± 0.0	1.7	56.9 ± 0.0	1.0	63.7 ± 0.2	7.8
pH 7.2		47.1 ± 0.2 ^a
	0.5	48.5 ± 0.1	1.4	48.7 ± 0.1	1.7	50.7 ± 0.0	2.1	63.8 ± 0.3	16.9
	1	49.4 ± 0.1	2.3	49.3 ± 0.0	2.2	52.0 ± 0.1	3.3	65.4 ± 0.1	18.4
	2	49.9 ± 0.1	2.8	50.1 ± 0.1	3.1	52.6 ± 0.1	3.9	67.2 ± 0.2	20.2
	5	52.5 ± 0.1	5.4	52.1 ± 0.1	5.0	53.6 ± 0.1	5.0	70.3 ± 0.2	23.4
	10	54.6 ± 0.1	7.5	53.6 ± 0.1	6.5	54.5 ± 0.0	5.8	72.6 ± 0.2	25.7

^a

mean T_m measured for GCase at indicated pH value in the absence of inhibitor.