Table 1.
Identification and biological activity of endophytic fungi isolated from A. macrosperma a.
| GenBank acc. no. | Endophytic fungi code | Identified speciesc | % Similarity | Cytotoxic activity LC50 (μg/mL) | Antitumor activity LC50 (μg/mL) | ||||
|---|---|---|---|---|---|---|---|---|---|
| HepG2 | MCF7 | A549 | SGC-7901 | HeLa | |||||
| JN596334 | AM01 | Acremonium furcatum (HQ637291) | 100 | 325.72 (198.00~792.90) | 87.75 (45.86~165.40) | 88.70 (38.94~133.34) | 85.77 (54.82~176.32) | >100 | 99.08 (25.62~383.20) |
| JN596335 | AM02 | Acremonium furcatum (JF311939) | 100 | 206.31 (126.20~437.21) | 81.05 (56.32~120.06) | 76.29 (28.74~165.30) | 70.98 (36.26~98.87) | 92.56 (53.24~322.14) | 71.83 (57.64~89.51) |
| JN596336 | AM03 | Acremonium furcatum (DQ865092) | 99 | 262.94 (184.72~421.58) | 90.63 (65.52~142.30) | 88.65 (52.72~128.80) | 84.80 (53.73~128.73) | 94.07 (79.16~111.80) | 83.92 (62.20~131.03) |
| JN596337 | AM04 | Acremonium furcatum (DQ865092) | 100 | 291.30 (236.63~358.67) | 93.60 (85.27~127.54) | 91.98 (55.45~167.76) | 89.15 (44.08~122.66) | 97.74 (63.22~143.24) | 94.9 (65.72~157.74) |
| JN596338 | AM05 | Cylindrocarpon pauciseptatum (HQ441248) | 98 | >1000 | >100 | >100 | >100 | >100 | >100 |
| JN596339 | AM06 | Cylindrocarpon pauciseptatum (AB369414) | 100 | >1000 | >100 | >100 | >100 | >100 | >100 |
| JN596340 | AM07 | Trichoderma citrinoviride (HQ608144) | 100 | 4.86 (2.95~7.55) | 3.13 (2.26~5.72) | 3.07 (2.82~6.01) | 2.00 (1.89~5.43) | 3.44 (2.08~5.63) | 2.88 (2.14~6.37) |
| JN596341 | AM08 | Trichoderma citrinoviride (HQ608144) | 99 | 35.88 (34.02~37.84) | 24.63 (12.34~50.77) | 22.10 (15.85~31.17) | 16.22 (10.45~27.31) | 23.66 (20.22~44.38) | 22.08 (10.28~53.24) |
| JN596342 | AM09 | Trichoderma citrinoviride (AF414343) | 98 | 21.20 (11.10~32.20) | 11.03 (4.29~19.72) | 10.64 (7.70~20.10) | 8.83 (4.42~14.09) | 12.57 (9.41~18.09) | 10.11 (5.82~24.40) |
| JN596343 | AM10 | Paecilomyces marquandii (FR799470) | 100 | >1000 | >100 | >100 | >100 | >100 | >100 |
| JN596344 | AM11 | Chaetomium globosum (JF826003) | 99 | 7.71 (7.30~8.12) | 3.87 (2.96~5.45) | 3.92 (2.74~7.14) | 3.14 (2.27~6.62) | 4.05 (3.01~6.29) | 3.86 (2.87~7.33) |
| JN596345 | AM12 | Ascomycete Incertae sedis sp. (HQ623458) | 100 | 124.54 (67.51~183.57) | 53.77 (21.10~82.23) | 48.63 (23.11~61.76) | 46.92 (21.10~76.50) | 74.77 (64.83~86.23) | 45.47 (18.89~67.71) |
| JN596346 | AM13 | Ascomycete Incertae sedis sp. (AM944361) | 99 | 228.32 (170.54~311.08) | 75.70 (69.05 ~82.99) |
75.22 (69.59~81.30) |
58.21 (39.79~85.15) | 85.39 (35.62~119.04) | 69.14 (23.66~108.74) |
| JN596347 | AM14 | Ascomycete Incertae sedis sp. (HQ623458) | 99 | 179.09 (131.23~243.95) | 46.64 (31.10~62.29) | 50.33 (42.20~60.02) | 47.47 (20.03~59.99) | 68.10 (29.74~153.40) | 44.43 (30.07~52.90) |
| JN596348 | AM15 | Ascomycete Incertae sedis sp. (AF413049) | 95 | 490.79 (258.30~682.34) | >100 | >100 | 89.35 (63.32~112.31) | >100 | 98.26 (55.40~182.31) |
| JN596349 | AM16 | Ascomycete Incertae sedis sp. (AM944361) | 97 | 61.57 (36.53~103.61) | 31.83 (10.98~53.26) | 35.88 (34.02~37.84) | 28.93 (20.15~33.29) | 39.04 (33.19 ~45.92) |
33.36 (26.41~42.15) |
| JN596350 | AM17 | Ascomycete Incertae sdis sp. (GU067747) | 100 | 14.88 (14.05~15.81) | 7.90 (5.33~20.15) | 8.59 (5.27~16.21) | 6.79 (4.63~12.28) | 10.04 (6.29~14.95) | 8.53 (6.22~19.65) |
| / | Podophyllo-toxinb | / | / | 2.72 (2.38~3.50) | 0.94 (0.66~2.03) | 1.02 (0.75~1.99) | 0.75 (0.58~1.77) | 1.37 (0.98~2.23) | 0.84 (0.62~1.52) |
a All determinations were done in triplicate, 95% confidence limits in parentheses. No mortality with negative control group (1% DMSO).
b Positive control group; c Closest identified relatives were shown in brackets (by BLAST search).