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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Nature. 2011 Dec 1;480(7375):118–122. doi: 10.1038/nature10598

Figure 1. EGF induces the PKM2–β-catenin interaction in the nucleus.

Figure 1

a, U87/EGFR cells were treated with or without EGF for 10 h.

b, U87/EGFR cells with or without PKM2 depletion were plated and counted 7 days after seeding. Data represent the means ± SD of three independent experiments.

c, e, U87/EGFR cells with or without PKM2 depletion were treated with or without EGF for 24 h (c) or 10 h (e).

d, U87/EGFR cells with or without PKM2 depletion were transfected with TOP-FLASH or FOP-FLASH, which was followed by EGF treatment for 10 h. Data represent the means ± SD of three independent experiments.

f, Myc-TCF4 was immunoprecipitated from PKM2-depleted or PKM2-undepleted U87/EGFR cells treated with or without EGF for 10 h.

g, h, PKM2 (g) or β-catenin (h) was immunoprecipitated from the indicated cell fractions of U87/EGFR cells treated with or without EGF for 6 h.

i, β-catenin immunoprecipitated from U87/EGFR cells with or without EGF treatment for 6 h was incubated with or without CIP (10 unit) for 30 min at 37°C followed by PBS washing for three times.

j, k, U87/EGFR cells stably expressing FLAG-tagged WT PKM2, PKM2 K433E (j), or PKM2 K367M (k) were treated with or without EGF for 6 h.