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Top row: Representative two-dimensional class average of negatively stained protein-containing nanodiscs (left) and macrodiscs (right). Each side of the display panels is 57.2 nm. Bottom row: 31P NMR spectra of the phospholipids in protein-containing nanodiscs (left) and macrodiscs (right) at 40°C. The molar ratios of DMPC to the 14-residue peptide are 1.67 for the nanodiscs and 13.3 for the macrodiscs.
