Figure 6. Immunohistochemistry for PrP^Sc in neuroendocrine tissues in Syrian golden hamsters infected with chronic wasting disease.

Pancreas (A through G) and adrenal gland (H through K) from mock (C, G, K) and WST CWD-infected (A, B, D, E, F, H, I, J) hamsters. Panels D through F, and H through J are the same field of view, which are separated into three panels according to the immunofluorescence staining. Pancreas was analyzed by dual immunofluorescence for synaptophysin (D, F, G) and PrP^Sc (E, F, G) while the adrenal gland was analyzed by dual immunofluorescence for PGP9.5 (H, J, K) and PrP^Sc (I, J, K) using laser scanning confocal microscopy. Serial sections of pancreas were also analyzed by hematoxylin and eosin (A) and PrP^Sc immunohistochemistry (B, C; brown punctate aggregates) and counterstained with hematoxylin. Scale bar in D and H is 10 µm and in A the scale bar is 100 µm.