Table 1. Incubation period following serial passage of CWD isolate into transgenic mice and Syrian golden hamsters.
| TgMo(sgh-PrP) | Syrian golden hamster | ||||||
| Recipient | Inoculum Number2 | Incubation Period, | Incubation Period, | ||||
| Group1 | (Incubation period) | days ± SEM | A/I3 | Pass No.4 | days ± SEM | A/I | Pass No. |
| M6148 | CWD (na) | 417±23.6 | 4/4 | First | |||
| Passage Line A: | |||||||
| M6298-99 | M6148.1 (369 d) | 198±3.1 | 5/5 | Second | |||
| H1429 | M6148.1 (369 d) | 389±0 | 3/3 | First | |||
| H1570 | H1429.3 (389 d) | 322±10.2 | 4/4 | Second | |||
| M6337 | M6298.1 (204 d) | 198±3.5 | 5/5 | Third | |||
| H1470 | M6298.1 (204 d) | 373±3.1 | 4/4 | First | |||
| M6339 | M6299.1 (192 d) | 189±5.7 | 4/4 | Third | |||
| H1471 | M6299.1 (192 d) | 379±3.0 | 4/4 | First | |||
| H1572 | H1471.4 (382 d) | 343±5.0 | 8/8 | Second | |||
| M6386 | M6339.2 (189 d) | 187±1.8 | 4/4 | Fourth | |||
| H1569 | M6339.2 (189 d) | 355±9.7 | 4/4 | First | |||
| Passage Line B: | |||||||
| M6332 | M6148.3 (473 d) | 360±16 | 3/3 | Second | |||
| M6374 | M6332.1 (343 d) | 160±4.6 | 4/4 | Third | |||
| M6388 | M6374.4 (151 d) | 157±0 | 3/3 | Fourth | |||
| H1604 | M6374.4 (151 d) | 477±15.45 | 3/3 | First | |||
Recipient rodents were either TgMo(sgh-PrP) indicated by a ‘M’ preceding the Recipient Group number or Syrian golden hamsters indicated by a ‘H’ preceding the Recipient Group number.
Inoculum was either a 10% brain homogenate (CWD isolate and M6148.1 inoculation of hamsters), a 1% brain homogenate (all other passages except one), or a 0.01% brain homogenate (M6148.3) from an individual animal in the Recipient Group (indicated by .1, .2, .3, or .4). The incubation period of the transgenic mouse or Syrian golden hamster used as inoculum is indicated in the parenthesis. Inoculation of M6148.1 into recipient group H1429 was by the intra-olfactory bulb route as previously described.
A/I, number affected with prion disease versus total number inoculated.
Number of serial passages in either TgMo(sgh-PrP) or Syrian golden hamsters. Hamsters were inoculated with a CWD isolate that was serially passaged into TgMo(sgh-PrP) for one, two, or three times.
In recipient group H1604, one hamster was found dead at 456 days post-inoculation and a second exhibited characteristics of old age. They did not exhibit overt symptoms of wasting disease or neurological dysfunction, but brain from these two SGH were positive for PrPSc. A third hamster was clinically positive for neurological disease and PrPSc in brain.