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. 2011 Dec 12;6(12):e28582. doi: 10.1371/journal.pone.0028582

Figure 4. A comprehensive siRNA sequence walk for the K12 mutation Leu132Pro.

Figure 4

This sequence walk shows the best siRNA was at position 9 (K12-L132P-9) which was very potent against the mutant reporter (pink line on plots) even at as low as 10 pM but did not significantly inhibit wild-type (blue line on plots) at concentrations as high as 6.25 nM. siRNA inhibitor K12-L132P-13 did not affect wild-type or mutant reporter while K12-L132P-2 and K12-L132P-3 knocked down both alleles to varying degrees NSC4 is a non-specific control siRNA, (negative control). siLUC is an siRNA specific for luciferase (positive control).