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. 2011 Dec 12;6(12):e28582. doi: 10.1371/journal.pone.0028582

Figure 6. Lack of off-target silencing of other keratins by lead inhibitor for K12 Leu132Pro mutation, siRNA K12-L132P-9.

Figure 6

SimplyBlue staining of cytoskeletal protein extracts from HaCaT cells revealed allele specific K12-L132P-9 siRNA had negligable effect on the range of endogenous keratins expressed in the HaCaT cell line. In contrast, a positive control siRNA directed against wild-type K6a demonstrated a dramatic decrease in K6a expression. Non-specific control siRNA NSC4 had no effect on keratin protein expression profile.